Evaluating of a Super Bright LED as a Spectrophotometer Light Source at The Clinical Laboratory

Rangga Santoso; Dyah Titisari; Prastawa ATP; Her Gumiwang Ariswati; Nora Bouzeghaia

doi:10.35882/jeeemi.v4i1.3

Rangga Santoso Teknologi Rekayasa Elektro-medis POLKESBAYA
Dyah Titisari Department of Electromedical Engineering, Poltekkes Kemenkes Surabaya
Prastawa ATP Department of Electromedical Engineering, Poltekkes Kemenkes Surabaya
Her Gumiwang Ariswati Department of Electromedical Engineering, Poltekkes Kemenkes Surabaya
Nora Bouzeghaia Department of Mechanical Engineering, Université Batna 2, Batna, Algeria

DOI: https://doi.org/10.35882/jeeemi.v4i1.3

Keywords: 530 nm LED, Lens, Cuvette, Photoresistors, Arduino, Absorption, Reagent, Biolyzer 100

Abstract

Spectrophotometers generally use a halogen lamp as a light source that passes through a filter (wavelength) according to the material to be analyzed. This study aims to analyze the ability of the LED as a light source on a spectrophotometer. In this study, the authors have determined blood sugar parameters as the test material. So that the determination of the wavelength of the LED as a light source must be adjusted to the specifications of the wavelength in the reagent manual procedure used. In the BAV Greiner Glucose Reagent procedure, the allowable wavelength is between 500 - 570 nm with a cuvette thickness of 1 cm. Measured against the reagent blank by the endpoint method. From this reference, the author uses an LED light source with a wavelength of 530 nm, Epistar brand green. The module in this study consisted of a 530 nm LED lamp as a light source, then a lens was added to focus the light beam from the 530 nm LED. The author also adds a Slit / Aperture or it can be called a small hole so that the light passing through is focused at one point of the circle and is passed to the cuvette. The results of the absorption of light will be received by the light sensor (photoresistor) and the data is processed by Arduino and the results are displayed on the display. From the results of this study, the value ranges error from 1% to 3% when a comparative test is carried out with the Analyticon type Biolyzer100 spectrophotometer with 6 different samples and is repeated 5 times each. From these data, it is found that the LED with a wavelength of 530 nm is effective as a light source for checking blood sugar.

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References

D. A. Pfeffer et al., “Quantification of glucose-6-phosphate dehydrogenase activity by spectrophotometry: A systematic review and meta-analysis,” PLoS Med., vol. 17, no. 5, pp. 1–18, 2020, doi: 10.1371/journal.pmed.1003084.

K. Mohammad, A. Zekry, and M. Abouelatta-Ebrahim, “LED Based Spectrophotometer can compete with conventional one,” Int. J. Eng. Technol., vol. 4(2), May 2015, doi: 10.14419/ijet.v4i2.4504.

R. Giovannetti, “The Use of Spectrophotometry UV-Vis for the Study of Porphyrins,” Macro To Nano Spectrosc., 2012, doi: 10.5772/38797.

J. S. Kim et al., “Simple LED spectrophotometer for analysis of color information,” Biomed. Mater. Eng., vol. 26, pp. S1773–S1780, 2015, doi: 10.3233/BME-151478.

S. Mandal and M. O. Manasreh, “An in-vitro optical sensor designed to estimate Glycated Hemoglobin Levels,” Sensors (Switzerland), vol. 18, no. 4, 2018, doi: 10.3390/s18041084.

P. J. Chen et al., “A practical portable photometer using LEDs as inspection light source,” I2MTC 2017 - 2017 IEEE Int. Instrum. Meas. Technol. Conf. Proc., pp. 1–6, 2017, doi: 10.1109/I2MTC.2017.7969714.

D. R. Albert, M. A. Todt, and H. F. Davis, “A low-cost quantitative absorption spectrophotometer,” J. Chem. Educ., vol. 89, no. 11, pp. 1432–1435, 2012, doi: 10.1021/ed200829d.

K. M. Zielinska-Dabkowska, J. Hartmann, and C. Sigillo, “LED light sources and their complex set-up for visually and biologically effective illumination for ornamental indoor plants,” Sustain., vol. 11, no. 9, 2019, doi: 10.3390/su11092642.

C. A. De Caro, “UV / VIS Spectrophotometry,” Mettler-Toledo Int., no. September 2015, pp. 4–14, 2015.

M. A. Al-Dhaheri, N. E. Mekkakia-Maaza, H. Mouhadjer, and A. Lakhdari, “Noninvasive blood glucose monitoring system based on near-infrared method,” Int. J. Electr. Comput. Eng., vol. 10, no. 2, pp. 1736–1746, 2020, doi: 10.11591/ijece.v10i2.pp1736-1746.

T. Pulli, T. Dönsberg, T. Poikonen, F. Manoocheri, P. Kärhä, and E. Ikonen, “Advantages of white LED lamps and new detector technology in photometry,” Light Sci. Appl., vol. 4, no. June, pp. 1–7, 2015, doi: 10.1038/lsa.2015.105.

T. Dönsberg et al., “Methods for decreasing uncertainties in LED photometry,” 17th Int. Congr. Metrol. CIM 2015, vol. 1, pp. 1–6, 2015, doi: 10.1051/metrology/20150011001.

A. B. D. Nandiyanto, R. Zaen, R. Oktiani, A. G. Abdullah, and L. S. Riza, “A simple, rapid analysis, portable, low-cost, and Arduino-based spectrophotometer with white LED as a light source for analyzing solution concentration,” Telkomnika (Telecommunication Comput. Electron. Control., vol. 16, no. 2, pp. 580–585, 2018, doi: 10.12928/TELKOMNIKA.v16i2.7159.

K. A. Mohammad, A. Zekry, and M. Abouelatta, “LED Based Spectrophotometer can compete with conventional one,” Int. J. Eng. Technol., vol. 4, no. 2, p. 399, 2015, doi: 10.14419/ijet.v4i2.4504.

P. Visconti, A. Lay-Ekuakille, P. Primiceri, G. Ciccarese, and R. De Fazio, “Hardware design and software development for a white LED-based experimental spectrophotometer managed by a PIC-based control system,” IEEE Sens. J., vol. 17, no. 8, pp. 2507–2515, 2017, doi: 10.1109/JSEN.2017.2669529.

N. Chaianantakul et al., “Development of mini-spectrophotometer for determination of plasma glucose,” Spectrochim. Acta - Part A Mol. Biomol. Spectrosc., vol. 204, pp. 670–676, 2018, doi: 10.1016/j.saa.2018.06.107.

A. Watane, J. Chabowski-carpino, C. Science, and T. Instruments, “GOLD : Glucose Optical LED Detection,” 2017.

D. T. M. Dinh, V. A. Truong, A. N. P. Tran, H. X. Le, and H. T. T. Pham, Non-invasive Glucose Monitoring System Utilizing Near-Infrared Technology, vol. 69. Springer Singapore, 2020.

N. A. M. Aziz, N. Arsad, P. S. Menon, S. Shaari, Z. M. Yusof, and A. R. Laili, “An assessment study of absorption effect: LED vs tungsten halogen lamp for noninvasive glucose detection,” J. Innov. Opt. Health Sci., vol. 8, no. 2, 2015, doi: 10.1142/S1793545815500133.

R. B. Dominguez, M. A. Orozco, G. Chávez, and A. Márquez-Lucero, “The evaluation of a low-cost colorimeter for glucose detection in salivary samples,” Sensors (Switzerland), vol. 17, no. 11, pp. 19–21, 2017, doi: 10.3390/s17112495.

P. Jain, R. Maddila, and A. M. Joshi, “A precise non-invasive blood glucose measurement system using NIR spectroscopy and Huber’s regression model,” Opt. Quantum Electron., vol. 51, no. 2, pp. 1–15, 2019, doi: 10.1007/s11082-019-1766-3.

B. Javid, F. G. Faranak, and F. S. Zakeri, “Noninvasive optical diagnostic techniques for mobile blood glucose and bilirubin monitoring,” J. Med. Signals Sens., vol. 8, no. 3, pp. 125–139, 2018, doi: 10.4103/jmss.JMSS-8-18.

C. Kalkbrenner, A. Van, T. Smith, and D. Charles, “Low-Cost Spectrophotometer,” pp. 1–17.

T. R. Dias and B. F. Reis, “A LED based photometer for solid phase photometry: Zinc determination in pharmaceutical preparation employing a multicommuted flow analysis approach,” J. Braz. Chem. Soc., vol. 23, no. 8, pp. 1515–1522, 2012, doi: 10.1590/S0103-50532012005000014.

S. Q. Jin, C. Huang, G. Xia, M. Y. Hu, and Z. J. Liu, “Bandwidth correction in the spectral measurement of light-emitting diodes,” J. Opt. Soc. Am. A, vol. 34, no. 9, p. 1476, 2017, doi: 10.1364/josaa.34.001476.

D. González-Morales, A. Valencia, A. Díaz-Nuñez, M. Fuentes-Estrada, O. López-Santos, and O. García-Beltrán, “Development of a low-cost UV-Vis spectrophotometer and its application for the detection of mercuric ions assisted by chemosensors,” Sensors (Switzerland), vol. 20, no. 3, 2020, doi: 10.3390/s20030906.

N. Demitri and A. M. Zoubir, “Measuring Blood Glucose Concentrations in Photometric Glucometers Requiring Very Small Sample Volumes,” IEEE Trans. Biomed. Eng., vol. 64, no. 1, pp. 28–39, 2017, doi: 10.1109/TBME.2016.2530021.

V. R. Pereira and B. S. Hosker, “Low-cost (<€5), open-source, potential alternative to commercial spectrophotometers,” PLoS Biol., vol. 17, no. 6, pp. 1–8, 2019, doi: 10.1371/journal.pbio.3000321.

A. Wego, “Accuracy simulation of an LED based spectrophotometer,” Optik (Stuttg)., vol. 124, no. 7, pp. 644–649, 2013, doi: 10.1016/j.ijleo.2012.01.005.

M. W. Prairie, S. H. Frisbie, K. K. Rao, A. H. Saksri, S. Parbat, and E. J. Mitchell, An accurate, precise, and affordable light emitting diode spectrophotometer for drinking water and other testing with limited resources, vol. 15, no. 1. 2020.

M.-E. Bouza, A. Nastou, C. Panigyraki, and C. Makedonas, “Introducing spectrophotometry in the school lab employing LEGO bricks and LEDs,” Chem. Teach. Int., vol. 1, no. 1, pp. 1–8, 2019, doi: 10.1515/cti-2018-0012.

D. S. R. . Rahayu, M. R. Mak’ruf, and S. Syaifudin, “Luxmeter Design with Proximity Sensor to Efficiently Test Light Intensity and Distance on Lamp Operation in Hospitals”, International Journal of Advanced Health Science and Technology, vol. 1, no. 1, pp. 20–25, Oct. 2021.